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vacuum degassing equipment  (Eppendorf AG)


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    Structured Review

    Eppendorf AG vacuum degassing equipment
    Vacuum Degassing Equipment, supplied by Eppendorf AG, used in various techniques. Bioz Stars score: 99/100, based on 200907 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/vacuum+degassing+equipment/Eppendorf/us12329800-310-7-10
    Average 99 stars, based on 200907 article reviews
    vacuum degassing equipment - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Evaporation:

    Article Title: Annexin-coated particles
    Article Snippet: Phosphatidylcholin (PC, 1,2-dioleoyl-sn-glycero-3-phosphocholine) and phosphatidylserine (PS, 1,2-dioleoyl-sn-glycero-3-phospho-L-serine) (both purchased from Avanti Lipids Polar) were mixed in a 5:1 ratio (PC:PS), supplemented with 50 μg/ml endotoxin-free OVA and solved in Chloroform (≥99.9%, SIGMA-Aldrich). .. After evaporation using a concentrator 5301 with vacuum degassing equipment (Eppendorf), the dry lipid/OVA-pellet was resuspended with DPBS (Ca2+ and Mg2+ included; Life Technologies) and sonicated with a SONOPULS HD 2070 device (Bandelin, at Cycle 5, 30% power) for 1 min. Lipid-vesicles were centrifuged for 30 min by 18,000×g at 4° C. After removing the supernatant, lipid-vesicles were resuspended with fresh DPBS. ..

    Article Title: Dectin-1 Binding to Annexins on Apoptotic Cells Induces Peripheral Immune Tolerance via NADPH Oxidase-2.
    Article Snippet: Phosphatidylcholine (PC, 1,2-dioleoyl-snglycero-3-phosphocholine) and phosphatidylserine (PS, 1,2-dioleoyl-sn-glycero-3-phospho-L-serine) (both purchased from Avanti Lipids Polar) were mixed in a 5:1 ratio (PC:PS), supplemented with 50 mg/ml endotoxin-free OVA (invivogen) and suspended in Chloroform (R99.5%, Sigma-Aldrich). .. After evaporation using a Concentrator 5301 with vacuum degassing equipment (Eppendorf), the dry lipid/OVA-pellet was resuspended in DPBS (Ca2+ and Mg2+ included; DPBS2+; Sigma-Aldrich) and sonicated with a SONOPULS HD 2070 device (Bandelin, at Cycle 5, 30% power) for 1 min. Lipid vesicles were centrifuged for 30 min by 18,000xg at 4 C. After removing the supernatant, lipid vesicles were resuspended in DPBS. ..

    Sonication:

    Article Title: Annexin-coated particles
    Article Snippet: Phosphatidylcholin (PC, 1,2-dioleoyl-sn-glycero-3-phosphocholine) and phosphatidylserine (PS, 1,2-dioleoyl-sn-glycero-3-phospho-L-serine) (both purchased from Avanti Lipids Polar) were mixed in a 5:1 ratio (PC:PS), supplemented with 50 μg/ml endotoxin-free OVA and solved in Chloroform (≥99.9%, SIGMA-Aldrich). .. After evaporation using a concentrator 5301 with vacuum degassing equipment (Eppendorf), the dry lipid/OVA-pellet was resuspended with DPBS (Ca2+ and Mg2+ included; Life Technologies) and sonicated with a SONOPULS HD 2070 device (Bandelin, at Cycle 5, 30% power) for 1 min. Lipid-vesicles were centrifuged for 30 min by 18,000×g at 4° C. After removing the supernatant, lipid-vesicles were resuspended with fresh DPBS. ..

    Article Title: Dectin-1 Binding to Annexins on Apoptotic Cells Induces Peripheral Immune Tolerance via NADPH Oxidase-2.
    Article Snippet: Phosphatidylcholine (PC, 1,2-dioleoyl-snglycero-3-phosphocholine) and phosphatidylserine (PS, 1,2-dioleoyl-sn-glycero-3-phospho-L-serine) (both purchased from Avanti Lipids Polar) were mixed in a 5:1 ratio (PC:PS), supplemented with 50 mg/ml endotoxin-free OVA (invivogen) and suspended in Chloroform (R99.5%, Sigma-Aldrich). .. After evaporation using a Concentrator 5301 with vacuum degassing equipment (Eppendorf), the dry lipid/OVA-pellet was resuspended in DPBS (Ca2+ and Mg2+ included; DPBS2+; Sigma-Aldrich) and sonicated with a SONOPULS HD 2070 device (Bandelin, at Cycle 5, 30% power) for 1 min. Lipid vesicles were centrifuged for 30 min by 18,000xg at 4 C. After removing the supernatant, lipid vesicles were resuspended in DPBS. ..



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